Journal: Redox Biology
Article Title: Adaptive responses of neuronal cells to chronic endoplasmic reticulum (ER) stress
doi: 10.1016/j.redox.2023.102943
Figure Lengend Snippet: Impaired lysosomes in TgR and TmR cells A. Immunofluorescence staining showed that LysoTracker red-positive ‘giant vesicular’ compartments were colocalized with LAMP2 and cathepsin D (CTSD) in untreated HT22 WT, TgR and TmR cells. DAPI (blue) was used to stain DNA. Scale bars: 20 μm. Values represent mean ± S.E.M., n = 3 (150–250 cells per group), ∗∗∗p < 0.001. B. Western blotting analysis displayed increases in immature forms of CTSD in TmR cells compared with HT22 WT cells. HSP90 was used as loading control. Values represent mean ± S.E.M., n = 3, ns non-significant, *p < 0.05 compared with the corresponding groups of the WT cells C. Relative cathepsin D/E activity of ER stress-resistant cells was reduced compared to HT22 WT cells. Values represent mean ± S.E.M., n = 3, ∗∗p < 0.01, ∗∗∗p < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Article Snippet: Cathepsin D/E substrate , Enzo Life Sciences , BML-P145 , Cathepsin D/E substrate.
Techniques: Immunofluorescence, Staining, Western Blot, Activity Assay